Objective To audit the best evidence on prevention and management of urinary retention after radical operation of cervical cancer, and to analyze the barriers to evidence-based nursing practice, so as to provide reference for clinical nursing. Methods The best evidences were searched and summarized using computer, and the audit indicators and methods were identified, then the obstacle factors were analyzed according to the audit results. Results Totally 13 quality audit indicators were developed according to the 12 best evidences. The obstacle factors mainly included training shortage, materials deficiency, awareness of medical staff, and lack of standardized management. Conclusion Clinical departments should select evidences according to the clinical environment, the factors promoting and hindering medical staff′s application of evidence, and the patients′ preferences. At the same time, the users need to update the evidences continuously.