Objective To explore the effect of online-offline and micro-digital interactive system based blended teaching in histological lab classes. Methods Totally 48 nursing students were randomly divided into a control group(n=24), who received traditional teaching method in histological lab classes, and an experimental group (n=24), who were subjected to online-offline and micro-digital interactive system based blended teaching. Then the learning effect of the two groups were evaluated from three aspects:learning process, lab reports and questionnaire. Results Compared with the control group, the students in the experimental group were more satisfied with 6 aspects (as in learning interest) of the results regarding the online-offline and micro-digital interactive system based blended teaching (P<0.05,P<0.01). Meanwhile, the scores of online test and lab experiment test, and the number of tissue sections observed in the experimental group were significantly higher than those in the control group(P<0.01 for all). Conclusion Online-offline and micro-digital interactive system based blended teaching can effectively improve the effect of lab teaching of histology, and train students′self-directed learning abilities.