Objective To find and apply the best evidence on position management of pediatric prone position operations, and to reduce the complications in prone positions. Methods From May to December 2019, by searching the relevant best evidences, we formulated 23 audit criteria.Then we set up corresponding evidence-based practice program, utilized JBI clinical evidence practice system, then integrated the best evidences into nursing practice, and finally conducted the post-intervention audit.A 2-round quality examination was conducted in the operation room. Results When compared with the baseline examination, nurses′ knowledge on position placement and pressure sore prevention and protection improved significantly,and the implementation rate of the 12 audit criteria significantly improved, and the incidence of skin injury in children decreased significantly (P＜0.01 for all). Conclusion Application of the best evidence on position management of prone position operations, can improve nurses′ cognition of position placement knowledge and pressure sore prevention and protection knowledge, and reduce the incidence of skin injury.