Objective To explore the application effect of interprofessional simulation teaching in the course of Nursing Planning & Implementation. Methods Totally 37 and 30 undergraduate nursing students enrolled in 2015 and 2016 were taken as a control group and an experimental group. During the 16 teaching hours experimental teaching in the course of Nursing Planning & Implementation, the control group was taught using routine simulation teaching, while the experimental group received interprofessional simulation teaching by recruiting 10 undergraduate students majored in clinical medicine (5 students) or pharmacy (5 students). Results After the teaching, the total score of teamwork attitudes, the three sub-dimensions of situational monitoring, mutual support and communication of the experimental group were significantly higher than those of the control group, and the two sub-dimensions of situational monitoring and mutual support in teamwork perceptions were significantly higher than those of the control group (P＜0.05,P＜0.01). There was no significant difference in satisfaction with simulation experience between the two groups (P＞0.05). Conclusion Interprofessional simulation teaching can improve nursing undergraduate students′ attitudes towards team colla-borative practice and their perceptions of team cooperation.