Objective To explore the effect of counterpart assistance on improving nursing service quality for a grass-root hospital. Methods We established assistance organization and management system, and understood the demand of the grass-root hospital, then chose and sent nursing administration experts and nursing specialists to guide the hospital in site, trained nurses in the assisted hospital and accepted the nurses study in our hospital, constructed high quality nursing service system, and conducted distance training and consultation. Results The assisted hospital was rated as a secondary excellent hospital, a 2A hospital and a baby-friendly hospital, and the nursing in the hospital was recognized as state level key nursing specialty. Conclusion Conducting counterpart assistance for grass-root hospital is benefit to improve nursing administration and nursing specialty level for the assisted hospital, then enhance their nursing service quality.