Objective To develop the Information Literacy Self-rating Scale for Clinical Nurses, and to provide an assessment tool for clinical nurses′ information literacy. Methods Based on concept defining and literature analysis, the item pool of the Information Li-teracy Self-rating Scale for Clinical Nurses was formed, and a draft scale was formed after a 2-round expert consultation and a small sample pilot study, which was then used to investigate 447 clinical nurses, the data of the 227 nurses were utilized for item analysis and reliability and validity test, and the data of the other 220 nurses were used for confirmatory factor analysis. Results The scale consisted of 35 items with 3 dimensions: information consciousness, information ability, information security and ethics. The Cronbach′s α coefficient of the scale was 0.974, and its split-half reliability coefficient was 0.855. The content validity index of the scale was 0.900, and the content validity index of each item was 0.857-1.000. Exploratory factor analysis showed that 3 common factors were extracted, which could explain 72.100% of the total variance.The χ2 /df of confirmatory factor analysis model was 2.041, the Comparative Fit Index was 0.953, Tucker Lewis Index was 0.961, the Standardized Root Mean Square Residual was 0.048, and the Root Mean Square Error of Approximation was 0.040. Conclusion The Information Literacy Self-rating Scale for Clinical Nurses has good reliability and validity, and it can be used as a self-rating scale measuring information literacy level of cli-nical nurses.