Objective To investigate the status of nursing interns′ transformation impact and feedback seeking behavior, and to analyze the influencing factors of nursing interns′ feedback seeking behavior. Methods Using cluster sampling method, 189 nursing interns working in general hospitals were selected to fill out the general information questionnaire, the Transformation Impact Assessment Scale and Feedback Seeking Behavior Scale. Results Nursing interns′ transformation impact score was (92.66±19.71) points, and feedback seeking behavior score was (61.27±9.35) points. Such factors as whether being a student leader, attitudes towards nursing work and transformation impact contributed to influence nursing interns′ feedback seeking behavior (P＜0.01 for all). Conclusion Transformation impact and feedback seeking behavior of nursing interns were at medium level, suggesting that nursing educators and managers should create a good feedback interactive cultural atmosphere, adjust the influencing factors of feedback seeking behavior of nursing interns, correct their attitudes towards nursing work, minimize the transformation impact, in an attempt to improve the feedback seeking behavior of nursing interns and improve the quality of clinical internship.