Objective To explore the relationship between humanistic practice ability and psychological capital of nurses during the standardized training period. Methods A total of 955 nurses during the standardized training period were investigated using the general information questionnaire, Nurse Humanistic Practice Ability Scale and Nurse Psychological Capital Scale(Chinese version). Results The total score for humanistic practice ability was (111.45±16.11) points， and that for psychological capital was (95.25±15.48) points, with the 2 scores having a positive correlation (r=0.697，P＜0.01). A hierarchical regression analysis showed that, service length, concerns from family, self-efficacy and hope of psychological capital were the main factors influencing humanistic practice ability of nurses during the standardized training period （P＜0.05,P＜0.01）, and psychological capital could explain 50.4% of the total variance of humanistic practice ability. Conclusion The humanistic practice ability and psychological capital of nurses during the standardized training period are both at medium level, and the two variables enjoy a positive correlation. Nurses managers should ramp up training from perspective of psychological capital to enhance the humanistic practice ability of nurses during the standardized training period.