Objective To explore the risk factors associated with coronavirus infection in healthcare workers, and to provide evidence-based infection prevention and control protocol for them. Methods Papers published from 2002 to February 2020 were searched in electronic databases.Stata 12.0 was employed for meta-analysis. Results Eleven publications were included involving 4 795 healthcare workers. Meta-analysis showed that such high-risk procedures as tracheal intubation ［OR=4.231，95%CI（1.625,11.015）］, tracheotomy［OR= 5.069，95%CI（2.437,10.543）］,Suctioning［OR=1.672，95%CI（1.349，2.072）］, non-invasive ventilation［OR=3.140，95%CI（1.439,6.851）］, and chest compression ［OR=4.500,95%CI（1.500,13.800）］ were risk factors for coronavirus infection in healthcare workers. Conclusion Performing high-risk treatment procedures increases infection risk in healthcare workers, so they should be vigilant and actively take preventive measures during these procedures.