Objective To enhance nurses′ cognition on Advance Care Planning (ACP), and to improve the implementation of ACP. Methods The ACP related training contents and forms were designed based on the Comskil Communication Model, ACP Theoretical Model and systematic literature review, then 110 nurses received ACP communication training through "Xinglin Wan Yu" video, ACP knowledge education booklet, "my end-of life" booklet, etc. The nurses′ knowledge, attitude and self-efficacy on ACP were assessed before and after the training. Results After the training, the nurses′ accuracy rate on ACP cognition improved, and the accuracy rate for two items (ACP communication contents and realizing treatment preferences will be respected) improved significantly when compared with those before the training (P<0.05,P<0.01). The scores of nurses′ attitude towards ACP decreased, and two items decreased significantly (P<0.05 for both), and six items of self-efficacy scored significantly different before and after the training (P<0.05,P<0.01). Conclusion Conducting ACP communication training for nurses can enhance their cognition on ACP to some extent, improve their attitude towards ACP changing positively, thus it is conducive to pushing ACP implementation in clinical nursing.