Objective To evaluate the application effect of the six-step cancer information disclosure model in disease information disclosure for prostate cancer patients with positive puncture biopsy. Methods A total of 98 prostate cancer patients with positive puncture biopsy were randomly divided into an intervention group and a control group, with 49 patients in each group. The disease information of the control group was disclosed using the conventional method, while the patients in the intervention group were informed using the six-step cancer information disclosure model. Results After disease information disclosure, the total score of the self-acceptance scale of the intervention group was significantly higher than that of the control group, and the total score of negative affect and death anxiety were significantly lower than those of the control group (P<0.01 for all). Conclusion Application of the six-step cancer information disclosure model in prostate cancer patients with positive puncture biopsy, can improve patients′ self-acceptance level, reduce their negative affect and death anxiety, which is significant for the patients.