Objective To establish a feeding program for preterm infants based on the best evidence, and to explore its clinical application effect. Methods According to the standard procedures of JBI Model of Evidence Based Healthcare, the best evidence of feeding strategies for preterm infants was obtained. Then the 96 preterm infants admitted to hospital from January to March 2019 were taken as a control group, and the 129 preterm infants admitted to hospital from May to July 2019 were regarded as an intervention group. The control group received routine feeding care, while the intervention group received the feeding strategies for preterm infants based on the best evidence. The rate of breast-feeding during hospitalization, the incidence of feeding intolerance, the time of starting oral feeding, the time of full enteral feeding and the length of hospitalization were observed. Results The rate of breast-feeding in the intervention group was significantly higher than that of the control group, incidence of feeding intolerance was significantly lower than that of the control group, and length of hospitalization was significantly shorter than that of the control group (P<0.05，P<0.01). There were no significant differences in the time of starting oral feeding and reaching full enteral feeding between the two groups (P>0.05 for both). Conclusion Practice of feeding strategies for preterm infants based on the best evidence, could standardize nurses′ feeding behavior, increase the rate of breast-feeding, and reduce the incidence of feeding intolerance, then improve the nursing quality for preterm infants.