Objective To understand workplace bullying and its influencing factors among clinical nurses, and to provide reference for prevention, management and education. Methods Totally 543 clinical nurses from 5 hospitals in Beijing were investigated using Chinese version of Negative Acts Questionnaire-Revised (NAQ-R). Results The clinical nurses′ total NAC-R score was (31.24±10.73), totally 215（39.6%） nurses experienced workplace bullying in the past 6 months, and 12（2.2%） of them suffered severe bullying. Regression analysis indicated that, working years, educational background, employment mode and hospital level were major influencing factors of nurses′ workplace bullying （P＜0.05,P＜0.01）. Conclusion Workplace bullying is common among clinical nurses. Those nurses employed in tertiary hospitals, with advanced education, contract system and worked for 11-15 years are prone to suffer from workplace bullying.Nursing managers and educators should pay more attention to conduct effective preventive measures and targeted education, so as to reduce nurses′ workplace bullying.