Objective To understand the current situation and influencing factors of workplace bullying among nursing interns, and to provide reference for developing targeted intervention dealing with workplace bullying. Methods A total of 315 nursing interns were recruited using convenience sampling, then they were investigated utilizing the Chinese version of the Negative Acts Questionnaire. Results The nursing interns′ score of workplace bullying was 31.43±9.92, and 107 (33.97%) students suffered from workplace bullying in the past 6 months. The students with different educational background, places of origin, average academic performance, and reasons for selecting nursing major scored significantly different in workplace bullying (P<0.01 for all). Multiple regression analysis showed that, reason for selecting nursing major, place of origin and educational background were main influencing factors of nursing interns′ perceived workplace bullying (P<0.05, P<0.01). Conclusion The rate of perceived workplace bullying among nursing interns is relatively high, which should be paid attention by nursing managers and educators, and effective preventive measures and targeted education should be given to reduce workplace bullying for nursing interns.