Objective To develop an electronic information system for hemodialysis and to evaluate its application effect. Methods The electronic information system for hemodialysis was developed based on standard blood purification procedure to establish closed-loop work process. And the system was connected with hospital information systems. Qualified rate of nursing documentation, nurses′ completion of designated patient′s treatment and recording working hours, as well as patient satisfaction with nursing work were compared before and after application of the system. Results The qualified rate of nursing documentation and patient satisfaction were significantly enhanced after application the system (P<0.01 for both). Nurses spent less time on completion of dialysis treatment for 5 designated patients and on recording after application of the system (P<0.01 for both). Conclusion The development and application of electronic information system for hemodialysis based on clinical practice is conducive to improving work efficiency of nurses and nursing quality, thus to enhance patient satisfaction.