Objective To apply the best evidence regarding pain management after pelvic floor reconstruction into clinical practice, thus to relieve postoperative pain of patient and to enhance medical staff′s awareness of and attention to pain management. Methods Literature regarding pain management after pelvic floor reconstruction for the treatment of pelvic dysfunction were retrieved and the quality of the literature was evaluated, then fourteen best evidence were concluded. Combining actual clinical situation and professional judgment,12 audit criteria were established. Then the evidence were implemented into clinical practice involving 30 patients and 20 nurses in baseline audit and re-audit respectively. Results The postoperative intensity was significantly milder, whereas patient satisfaction with pain management, autonomy of analgesic behavior and knowledge of pain management, as well as nurse′s knowledge of pain management were significantly higher at the re-audit compared with the baseline audit (P<0.05, P<0.01). The rate of personalized pain management regimen made by physician in collaboration with nurse increased from 53.3% to 100%, and the rate of combining use of pharmacological and non-pharmacological interventions increased from 10.0% to 53.3%(P<0.01 for both). Conclusion The implementation of best evidence into clinical practice can standardize nurse′s behavior, thus to alleviate postoperative pain of patient after pelvic floor reconstruction, and improve patient′s positive experience of postoperative analgesia and self-efficacy.