Objective To investigate the level of and factors associated with relocation stress among family members of patients transferred from CCU to ward, and to provide reference for targeted intervention. Methods Totally, 114 family members of patients transferred from CCU to ward were investigated using a general information questionnaire, the Family Relocation Stress Scale, the Mishel′s Uncertainty of Illness Scale-family member version and Caregiver Burden Inventory. Results The participants scored 38.07±7.28 on relocation stress, 74.15±8.47 on caregiving burden, and 77.93±6.95 on uncertainty of illness. Gender, education background, kinship, family income, medical payment, CCU stay time, illness uncertainty and caregiving burden were predictors of relocation stress of family members (adjusted R2=0.552;P<0.05, P<0.01). Conclusion Family members of patients transferred from CCU to ward experience severe caregiving burden and uncertainty of illness, obvious relocation stress which is affected by several factors. Medical staff should pay attention to this population, strengthen communication and relieve their worry, thus to facilitate patient′s transitions and further treatment.