Objective To improve the quality and efficiency of intraoperative care in otologic surgery. Methods Four hundred patients underwent otologic surgery were divided into two groups of 200 chronologically, receiving either routine intraoperative care (the control group) or alternative care that followed the standard operating procedures (the intervention group). The application effects in cochlear implantation, middle ear surgery, acoustic neuroma surgery and balloon dilation eustachian tuboplasty were compared. Results The intervention group required shorter time for the former three surgeries, however, had lower incidence of equipment malfunctioninginduced interruptions in the surgery compared with the control group (P<0.05, P<0.01). The surgeons′ satisfaction with nursing work in the intervention group was significantly higher than the control group (P<0.01 for all). Conclusion The SOP for standardization of intraoperative care in otologic surgery facilitates to provide homogeneous care, thus to improve the efficiency and quality of operating room nursing.