Objective To improve cleaning staffers′ awareness of keeping frequently touched objects clean, and enhance the cleanliness of frequently touched objects in the vicinity of patients. Methods A transtheoretical model was used to intervene the work behaviors of 88 cleaning staffers in a tertiary hospital of traditional Chinese medicine in Zhangjiagang City.The behavioral stages of cleaning staffers were assessed by the researchers; then the staffers were divided into 5 groups according to the behavioral stages they were at, and received stage-specific training and guidance.Before and 6 months after intervention, behavioral stage distribution of cleaning staffers, the clearance rate of fluorescent markers and the qualified rate of adenosine triphosphate （ATP） biofluorescence detection on the surface of frequently touched objects in the vicinity of patients were compared. Results Six months after intervention, behavioral stage distribution of cleaning staffers, the clearance rate of fluorescent markers and the qualified rate of ATP biofluorescence detection on the surface of frequently touched objects in the vicinity of patients were significantly improved, as compared to before （P＜0.01 for all）. Conclusion Training and intervention based on transtheoretical model can improve cleaning staffers′ awareness of keeping frequently touched objects clean, make them change their attitudes towards and behaviors of cleaning work, and help standardized cleaning and disinfection work process, thus improving the efficiency of cleaning and reduce the number of bacterial colonies on the surface of objects.