Objective Through the application of the best evidence for deep venous thrombosis (DVT) prevention and management, to reduce incidence rate of DVT in spinal surgery patients. Methods By referencing to JBI model for evidence-based healthcare, we searched and summarized the best evidence for DVT prevention and management.Then we formed inspection indicators, made baseline inspections before application of the best evidence, took transformative measures to boost application of the best evidence, and made post-application inspections to evlaute the effect. Results After the application of the best evidence, the incidence rate of DVT in spinal surgery patients decreased from 2.90% to 0; medical staffers′ knowledge levels of DVT prevention and management, the implementation rate of bleeding risk assessment, physical and drug prevention, and health education were significantly improved from baseline levels （P＜0.01 for all）. Conclusion The application of the best evidence for DVT prevention and management can improve medical staff′s DVT prevention and management level, standardize their behaviors, and reduce the incidence rate of DVT.