Objective To investigate the effects of PICC insertion via middle segment of femoral vein in infants and toddlers with difficult venous access（DIVA）. Methods Totally, 102 DIVA infants and toddlers received PICC insertion via middle segment of femoral vein. The clinical data involving insertion, and complications associated with catheter placement and indwelling were summarized and analyzed. Results The success rate of catheterization was 100%.The indwelling time ranged from 8 to 307 days. The rate of catheter tip reaching the optimal position was 85.29%.There was no perforation of artery during insertion, though the needle grazing the artery did occur in 2 cases (1.96%). The incidence rate of primary malposition was 5.88%, and the incidence rate of complications during PICC indwelling was 23.53%. Eighty-nine children (87.25%) received PICC removal as planed after completion of the treatment, while thirteen children (12.75%) experienced unplanned removal. Conclusion The technique of PICC insertion via middle segment of femoral vein in DIVA infants and toddlers has high success rate. It can meet the mid-and long-term infusion need of DIVA infants and toddlers.