Objective To analyze the usage of first-aid medicines from resuscitation carts, and to provide reference for standardizing preparation of first-aid medicines in resuscitation carts. Methods Firstaid medicines obtained through resuscitation carts for resuscitation were collected in 35 specialty departments from 2016 to 2018 in a tertiary hospital via the information management center, then data were analyzed to identify the annual consumption, use frequency, and use rate in different departments. Results The most frequently used medicines were adrenalin and noradrenalin within 3 years, but the most infrequently used were isoprenaline, aminophylline and promethazine. The use rate of half of the medicines on firstaid medicine list was less than 60%. There were significant correlations among the annual consumption, use frequency and use rate of each medicine (P<0.05 for all). Conclusion The amounts and kinds of essential firstaid medicines in resuscitation carts should be adjusted by considering specialty.