Objective To revise the WHO safe childbirth checklist, and to ensure the safety of maternal and infants. Methods A total of 852 puerperas giving birth in October 2017 were selected as a control group, who received routine nursing during perinatal period, and totally 886 puerperas delivered in October 2018 were selected as an observation group, who additionally received nur-sing intervention using the revised safe childbirth checklist. The occurance of postpartum complications and neonatal asphyxia and infection between the two groups were compared. Results There were significant differences in incidence of postpartum hemorrhage, infection and operative vaginal delivery between the two group （P＜0.05 for all）. The incidence of neonatal asphyxia and infection in the observation group were lower than those in the control group, while there were no significant differences （P＞0.05 for both）. Conclusion Application of the revised safe childbirth checklist is conducive to reducing the incidence of operative vaginal delivery and maternal and neonatal related complications, and ensure the safety of maternal and infants.