Objective To explore the effect of the intervention using graduated compression stocking on prevention of intradialytic hypotension for hemodialysis patients. Methods A cross matching design was utilized, and 18 maintennance hemodialysis patients were assigned to a control group （routine nursing intervention） and an intervention group （intervention using graduated compression stocking） crossover for intervention. The patients′ blood pressure and heart rates immediately before dialysis, 1 hour, 2 hours and 3 hours after dialysis, at the end of dialysis and 15 minutes stopping dialysis were measured, and their amount of residual ultrafiltration and fluid replenishment after dialysis were also measured. Results The intervention group suffered 29 case times intradialytic hypotension, and the control group encountered 37 case times. The systolic blood pressure at 1 and 2 hours after dialysis of the intervention group were significantly higher than those of the control group, their heart rates were significantly lower than those of the control group (P＜0.05 for all); there were no significant differences in blood pressure between the two groups at 3 hours after dialysis, at the end of dialysis and 15 minutes stopping dialysis (P＞0.05 for all); and the amount of residual ultrafiltration and fluid replenishment of the intervention group were significantly less than those of the control group (P＜0.05 for both). Conclusion The intervention using graduated compression stocking during dialysis for hemodialysis patients could prevent intradialytic hypotension to some extent, reduce the amount of residual ultrafiltration and fluid replenishment during dialysis, and guarantee the treatment effect of hemodialysis.