Objective To verify the effectiveness and feasibility of the prevention programme for methicillin resistant staphylococcus aureus ventilator associated pneumonia(MRSAVAP). Methods Totally 62 patients in ICU were divided into a control group and an observation group chronologically, with 31 patients in each group. The control group was given routine ICU nursing, while the observation group was taken Ottawa evidence transformation mode as theoretical framework, the MRSAVAP prevention programme was formulated and applied in clinical practice based on previous study. Results After the training, medical staff's knowledge on MRSAVAP prevention was significantly higher than that before the training (P<0.05). After the intervention, 2 patients (6.45%) in the observation group encountered MRSA colonization, which decreased to 0 after decolonization. The incidence of MRSAVAP, the time of mechanical ventilation and costs in ICU of the observation group were significantly lower than those of the control group (P<0.05, P<0.01）. Conclusion Application of the MRSAVAP prevention programme is conducive to improving medical staff′s knowledge on MRSAVAP prevention, decreasing ICU patients′ incidence of MRSAVAP, shortening the time of mechanical ventilation and reducing ICU costs.