Objective To investigate the effect of modified herringbone positioning in gynecological laparoscopic surgery. Methods A total of 100 cases of gynecological laparoscopic surgery were randomly and evenly divided into a control group, who were placed in routine lithotomy position, and an observation group, who were placed in modified herringbone position with the aid of a new type of position pads (positioners). The time of position placement, blood pressure fluctuations, and postoperative complications were compared between the two groups. Results The observation group had shorter time of position placement, and narrower blood pressure fluctuations, and higher proportion of patients with stable position during the surgery than the controls （P＜0.05,P＜0.01）. The satisfaction rate of doctors and nurses with the modified herringbone positioning was significantly higher than the routine methods (P＜0.05,P＜0.01). Conclusion The application of the modified herringbone positioning in gynecological laparoscopic surgery can reduce the fluctuation of blood pressure, boost patient comfort, and guarantee safety of the surgery.