Objective To compare the effect of using intermittent inflation devices and medical elastic stockings during laparoscopic surgery on the prevention of deep vein thrombosis (DVT) in the lower extremities. Methods A total of 100 patients who wore medical elastic stockings during laparoscopic gastrointestinal tumor resection to prevent DVT with in the period from January 2016 to January 2017 were selected as the control group. Another 100 patients using an intermittent inflation device during the same kind of surgery from February, 2017 to February, 2018 were enrolled into the observation group. The incidence rates of DVT in the lower extremities, length of time of DVT, and the changes of coagulation/fibrinolysis index before and after surgery in the 2 groups were compared. Results The incidence rate of DVT in the observation group was lower, and the length of time of DVT was shorter, than those in the control group （P＜0.05,P＜0.01）.The values of PT and tPA-Ag in the observation group were significantly higher than those in the control group 2 h after the start of surgery （P＜0.01 for both）; the values of PT at 24 hours after the surgery in the observation group was significantly lower than the control group （P＜0.01）, while the values of ATTP, tPA-Ag had no significant difference between the 2 groups （P＞0.05 for all）. Conclusion Intraoperative intermittent inflation can effectively reduce the incidence rate of DVT in the lower extremities and improve the coagulation/fibrinolysis index.