Objective To understand preservice training needs and the associated factors in newly graduated nurses in private tertiary care hospitals, and to provide information for formulating scientific and practical preservice training program. Methods A selfdesigned questionnaire was filled out by 98 newly graduated nurses and 35 nursing managers in terms of their preservice training needs. Results The score of training needs of newly graduated nurses was (4.44±0.45). Nurses′ training needs were significantly different from head nurses′ perspectives in nursing safety, disease knowledge, professional skill, hospital culture, nursing etiquette, professional attitude, and attitudes toward education and service (P<0.05, P<0.01). Multiple regression analysis showed that age, professional title, education level, and career development expectation were predictors of training needs of newly graduated nurses (P<0.05, P<0.01). Conclusion Newly graduated nurses have high preservice training needs, while head nurses pay more attention to nurses′ training needs of hospital development. Those who are younger, who have lower professional title and higher education background, and those who have career planning, report higher training needs. Nursing managers should formulate comprehensive, systematic, targeted and hierarchical training programs.