Objective To explore the effect of applying Kotter′s organizational change model in standardized training of new graduate nurses. Methods Fiftytwo new graduate nurses in 2015 were treated as the routine group and received standardized training with routine teaching method, while 58 new graduate nurses in 2016 were served as the Kotter group, receiving standardized training with alternative teaching method that employed Kotter′s organizational change model and revised the training program. Theoretical knowledge, practical skill, patient satisfaction and post competence of nurses in both groups were compared after 24 months of training. Results The abovementioned parameters were significantly higher in the Kotter group compared with the routine group (P<0.01 for all). Conclusion Employing Kotter′s organizational change model in standardized training of new graduate nurses can enhance their post competence.