Objective To describe the level of patient safety behaviors among clinical nursing staff and to analyze the influencing factors. Methods A total of 420 nurses were investigated with the Patient Safety Behavior Scale in Medical Staff. Results The score of safety compliance behavior was 5.69±0.36, and that of safety involvement behavior was 5.44±0.61. Multiple linear regression analysis showed that professional title, work length and education level were predictors of safety compliance behavior; and work length and education level were predictors of safety involvement behavior （P<0.05,P<0.01）. Conclusion Nurses′ safety compliance behavior was better than safety involvement behavior. Nursing managers should focus on improving nurses′ safety involvement behavior, especially on the involvement of adverse event reporting and patient safety related education. It is also necessary to cultivate and use nurses by considering their professional title, work length and educational background, thus to enhance their safety behaviors and guarantee patient safety.