Objective To apply the best available evidence of safe pneumatic tourniquet use in orthopedic surgery of the limbs and to reduce complications. 〖WTHZ〗Methods Following the JBI model for evidencebased healthcare and the "evidencebased continuous quality improvement framework", we chose the best evidence for safe pneumatic tourniquet use and formulated 10 audit criteria. Then we conducted field observation, questionnaire survey and expert interview to identify barriers to the implementation of the evidence, to analyze the available resources and to formulate solutions. Results The implementation of the 10 audit criteria was significantly improved during reaudit compared with the baseline audit (P<0.01 for all). The incidence of skin injury immediately after the surgery decreased from 65.0% in baseline audit to 20.0% in reaudit (P<0.01). 〖WTHZ〗Conclusion The implementation of best evidence about pneumatic tourniquet use in operating room can standardize nurses′ practice, thus to reduce complications in patients undergoing orthopedic surgery and ensure patient safety.