Objective To find out the major stress sources of nurses pregnant with a second child and the influencing factors. Methods Chinese Nurses Stressor Scale was used to investigate 200 nurses pregnant with a second child and working in 10 general hospitals in Guangdong province. Results The mean stress level of nurses pregnant with a second child was （92.17±24.84）.the scores of the dimensions in descending orders were as follows:time allocation and workload （14.96±3.78）, patient care （30.21±8.92）, professional nursing care and aspects of work （18.09±5.00）, work environment and instruments & devices （7.57±2.64）, managerial work and interpersonal relationship （21.68±7.72）.Nurses′ dimension scores differed significantly by trimesters,the ages of the first child,career titles, positions, working years, departments, posts,employment forms, and monthly income （P＜0.05 for all）. Conclusion The stress level of nurses pregnant with a second child was high.Nursing managers should take measures tailored to the nurses to alleviate their stress levels and improve their physical and mental health.