Objective To develop an evidencebased practice protocol for intrathecal labor analgesia. Methods Using the Stetler Model of research utilization as theoretical framework, we developed draft protocol based on field observation, identification of focus problems, literature reviewing, and extraction of evidence and recommendations. Then expert consultation was conducted to identify recommendation grade and to formulate the final protocol. Results The protocol included seven dimensions and 41 recommendations（38 strong recommendations and 3 weak recommendations）：assessment and preliminary management of labor pain, commencement of intrathecal labor analgesia, preparation before intrathecal labor analgesia, monitoring and management during intrathecal labor analgesia, activity management, dietary management, and termination of intrathecal labor analgesia. Conclusion The development of evidencebased practice protocol for intrathecal labor analgesia facilitates the achievement of multidisciplinary team agreement, thus to provide foundation for clinical practice.