Objective To run microbiological test for standby ventilators in the ICU, to clarify the bacterial contamination on the surface of the ventilator panel and the air inlet port, and provide the basis for standardizing the terminal disinfection management of the ventilators. Methods Microbiological tests were performed from samples harvested from the inlet ports and operating panels of 28 standby ventilators from 9 ICUs.At the meantime, through on-site observation and interviews, researchers tried to figure out implementation of routine treatment measures regarding the standby ventilator panels and inlet ports. Results The unqualified rate of microbial cultures on the surface of standby ventilator panels was 70.37%, and the unqualified rate of microbial cultures on the inlet port was 10.71%;10(35.71%) standby ventilators used dust-proof masks, and 18(64.29%) ventilator inlet ports were protected by ventilator test tubes or rubber stoppers.The qualified rate in microbial cultures of ventilator panels covered with dust mask was significantly higher than that in uncovered group (P＜0.01). Conclusion The bacterial contamination on the surface of standby ventilator panels can not be neglected, and dust-proof masks can reduce contamination by pathogenic microorganisms on the panel surface of ventilator. Therefore, the standby ventilator panels and inlet ports should be routinely cleaned and disinfected, and covered with dust-proof masks in time; bacterial filters should be installed to mitigate the risk of bacterial contamination of the standby ventilators.