Objective To develop the Attitude Towards Research Scale for Nursing Undergraduates and to test its reliability and validity.Methods Based on the ABC theory of attitude, the initial scale was developed through literature analysis, semi-structured interviews, Delphi expert consultation, and a pilot survey.From September to October 2024, nursing students from two undergraduate medical colleges in Tianjin and two in Yunnan province were selected for a questionnaire survey to perform item analysis and reliability and validity testing of the scale.Results The scale includes 44 items across six dimensions:knowledge of scientific research, beliefs about scientific research, emotional experiences of scientific research aspirations, self-evaluation emotional expe-riences of scientific research, tendencies for learning scientific research knowledge, and tendencies for joining in scientific research activities.Exploratory factor analysis extracted six common factors, with a cumulative variance contribution rate of 70.071%.The scale-level content validity index was 0.968, and the item-level content validity index ranged from 0.857 to 1.000.Confirmatory factor analysis results showed a good model fit (χ2/df=1.604,RMSEA=0.048,TLI=0.952,IFI=0.955,CFI=0.955,GFI=0.809).The Average Variance Extracted (AVE) for each dimension ranged from 0.606 to 0.829, all exceeding 0.500, and the correlation coefficients between dimensions were all less than the square root of AVE.The overall Cronbach′s α coefficient of the scale was 0.966, with individual dimension Cronbach′s α coefficients ranging from 0.849 to 0.960.The split-half reliability was 0.838, and the test-retest reliability was 0.953.Conclusion The Attitude Towards Research Scale for Nursing Undergraduates demonstrates good reliability and validity, and can be used as a reliable assessment tool for measuring the attitude towards research among undergraduate nursing students.