Objective To investigate the current status of clinical nurses′ attitudes toward information security and analyze the inf-luencing factors, and to provide references for nursing managers to develop targeted intervention measures.Methods A convenience sampling method was employed to select 575 clinical nurses from six Grade A hospitals in Anhui Province from November 2024 to January 2025.The survey utilized a general information questionnaire, the Information Security Attitude Questionnaire or Nurses, the Nursing Informatics Competency Scale, and the Patient Privacy Scale.Results The total score of clinical nurses′ information security attitude scale was （84.40±11.25） points.Multiple linear regression analysis indicated that educational level, whether the nurse had received specialized training in information system security, nursing informatics competency, and awareness of patient privacy protection were significant influencing factors for clinical nurses′ attitudes toward information security (all P＜0.05).Conclusion Clinical nurses′ attitudes toward information security are at a moderately high level, but there is considerable room for improvement.Nursing managers should focus on the group of nurses with lower educational backgrounds and conduct targeted educational training.Various methods should be employed to enhance clinical nurses′ nursing informatics competency and awareness of patient privacy protection behaviors, thereby improving their attitudes toward information security.