Objective To understand the current situation and influencing factors of master′s degree nurses′ sense of overqualification, and to provide reference for the development of targeted management programs.Methods A total of 581 master′s degree nurses were selected from 48 tertiary hospitals nationwide using stratified random cluster sampling, and were surveyed using the ge-neral information questionnaire, 9-item Scale of Perceived Overqualification, Organizational Support Scale, and Three-Dimensio-nal Inventory of Character Strengths (TICS).Results The total mean score of master′s degree nurses′ sense of overqualification was (29.84±7.62); years of working experience, number of night shifts per month, work content, daily workload assumed, sense of organizational support, and character strengths were the influencing factors of master′s degree nurses′ sense of overqualification (all P＜0.05).Conclusion Master′s degree nurses have a high sense of overqualification, and managers should pay attention to cultivating this group′s character strengths and providing some organizational support to improve the current status of master′s degree nurses′ sense of overqualification and maximize their value.