Objective To systematically search, extract, summarize, and analyze the best evidence for pelvic floor rehabilitation exercises in women during pregnancy, and to provide a reference for pelvic floor rehabilitation exercise interventions. Methods Following the "6S" evidence pyramid model, top-down searches were conducted to retrieve relevant domestic and international databases on pelvic floor rehabilitation exercises for women during pregnancy, including clinical decisions, guideline, evidence summary, expert consensus, and systematic review. Literature screening and quality assessment were conducted independently by 2 researchers, and relevant evidence was summarized with professional knowledge. Results A total of 9 publications were included, comprising 3 guidelines, 2 expert consensus statements, 1 best practice report, and 3 systematic review. Twenty-seven pieces of evidence were summarized in 8 areas: exercise principles, exercise assessment and guidance, exercise types, exercise procedures, exercise frequency and duration, exercise intensity, exercise effectiveness and supervision, and exercise precautions. Conclusion The best evidence on pelvic floor rehabilitation therapy for pregnant women can provide evidence-based support for clinical healthcare professionals and rehabilitation therapists. Health care providers should select the best evidence to guide the practice of pelvic floor exercises in pregnant women based on clinical contexts and specific needs, aiming to enhance the pelvic floor muscle strength of pregnant women and improve their quality of life during pregnancy and postpartum.