Objective To explore the clinical application effects of a noninvasive wearable skin-interfaced system in blood supply monitoring for patients after microvascular anastomosis.Methods Patients after free flap transfer or single severed finger replantation were divided into a control group and an experimental group chronologically, with 110 patients in each group.The blood supply of the control group was monitored using the traditional clinical methods, while the experimental group was additionally assessed by utilizing a noninvasive wearable skin-interfaced system.The incidence, timely detection rate, misjudgment rate, and the missed diagnosis rate of the vascular crisis between the two groups were compared, and the satisfaction of medical staff towards the system was investigated.Results A total of 108 patients and 105 patients in the control group and experimental group completed the study.The timely detection rate of vascular crisis in the experimental group was significantly higher than that in the control group, and its misjudgment rate and missed diagnosis rate of vascular crisis were significantly lower than those of the control group （all P＜0.05）.Medical staff′s satisfaction towards the noninvasive wearable skin-interfaced system was 4.89-5.00 points.Conclusion The noninvasive wearable skin-interfaced system can provide objective and accurate evidence for medical staff assessing blood supply, which is conducive to detecting vascular crisis timely and accurately in patients after microvascular anastomosis.