Objective To explore the current status of psychological flexibility of operating room nurses and analyze its influencing factors, in order to provide reference for nursing managers to formulate targeted interventions.Methods Convenience sampling method was used to select 403 operating room nurses from 13 tertiary public hospitals in Guizhou Province from November 2023 to April 2024, and the general information questionnaire, the simplified Multidimensional Psychological Flexibility Scale （MPFI-24）, the Social Support Rating Scale （SSRS）, and the Emotion Regulation Questionnaire （ERQ） were used to survey the operating room nurses.Results The total score of MPFI-24 was （49.67±9.96） points.Psychological flexibility was positively correlated with social support （r=0.392，P＜0.001） and cognitive reappraisal （r=0.594，P＜0.001）, and negatively correlated with expressive suppression （r=-0.206，P＜0.001）.Gender, education level and whether liking operating room nursing work had statistical significance in the three models of hierarchical regression analysis （all P＜0.05）.After controlling the general data variables, the path analysis results showed that social support not only had a direct effect on psychological flexibility, but also had an indirect effect on psychological flexibility through cognitive reappraisal （both P＜0.05）.Conclusion Psychological flexibility of operating room nurses is at a moderate level and needs to be further improved.Nursing managers should pay special attention to the important effects of social support and emotional regulation on nurses′ psychological flexibility, and take targeted intervention measures to improve nurses′ psychological flexibility.