Objective To translate the Japanese version of Self-regulated Learning Strategy Scale for Undergraduate Nursing Students(SRLSS-NS) into Chinese, to verify its reliability and validity, and to provide a valid assessment tool for measurement of undergraduate nursing students′ ability to apply learning strategies in China. Methods Following Brislin′s translation model, the original scale underwent forward translation, back-translation, cross-cultural adaptation, and pre-testing to develop a preliminary Chinese version SRLSS-NS. Using a convenience sampling method, a questionnaire survey of 346 students from 3 colleges in Jiangsu province and Hubei province was conducted to assess the reliability and validity of the Chinese version scale. Results The Chinese version contained 12 items. Exploratory factor analysis identified three common factors, explaining 87.509% of the cumulative variance, with factor loadings above 0.70 for all items. Confirmatory factor analysis showed good model fit: χ2/df=2.307, CFI=0.977, TLI=0.970, RMSEA=0.076, and SRMR=0.027. Correlation coefficients with the dimensions of the College Student Learning Strategies Questionnaire ranged from 0.542 to 0.699. The scale′s S-CVI/Ave was 0.972, with item-level I-CVI ranging from 0.833 to 1.000; Cronbach′s α was 0.962, Spearman-Brown split-half reliability coefficient was 0.927, and test-retest reliability coefficient was 0.947. Conclusion The Chinese version SRLSS-NS has good reliability and validity, making it suitable for assessing self-regulated learning strategy for applying knowledge and skills in the Chinese context.