Objective To translate the Student Perceptions of an Online Course (SPOC) scale and test its reliability and validity in Chinese context. Methods The Chinese version SPOC scale was formed through translation, expert consultation, pilot survey, and cultural adaptation, etc. Then 399 nursing or relevant medicine students were surveyed with C-SPOC to test its reliability and validity. Results According to exploratory factor analysis, six factors were extracted from 22 items, including module design (4 items), course content (2 items), promotion strategy (4 items), interaction (10 items) and resources (3 items). The cumulative variance contribution rate was 80.527%. The results of confirmatory factor analysis showed that χ2/df=1.867,RMSEA=0.062,GFI=0.881,AGFI=0.843,NFI=0.933,TLI=0.961,IFI=0.968,and CFI=0.967. The content validity index of scale （S-CVI/Ave） was 0.994. Cronbach′s α coefficient of the scale was 0.970, and the Spearman-Brown split-half reliability coefficient is 0.943. Conclusion The Chinese version SPOC has good reliability and validity, and can be used as an assessment tool for nursing or medical students to evaluate the quality of online courses.