Objective To understand the status quo of enteral nutrition interruption（ENI） in children in the pediatric intensive care unit (PICU) and analyze the relevant influencing factors, thereby providing references for the formulation of intervention measures for ENI in children. Methods A total of 299 children from PICU of a specialized children′s hospital were selected as research subjects. Instances of ENI in the children were observed and the causes were probed. Then the children were divided into an ENI group and a non-ENI group to analyze the influence of ENI on clinical outcomes of the children. Results Out of the 299 children, 190 (63.5%) experienced ENI, amounting to 373 instances in total, with an average of 2.0 (1.0, 3.0) instances per child and a duration of 16.0 (8.0, 29.3) hours per interruption. Feeding intolerance was the most common cause for interruption. Children in the ENI group had significantly longer mechanical ventilation duration, higher incidence rate of multi-organ dysfunction, longer PICU stay, and higher PICU hospitalization costs, as compared to the non-EN group (all P<0.05). Conclusion The incidence of ENI in children in the PICU is high and affects their outcomes. Intervention strategies for ENI should be developed to shorten the duration of ENI and reduce unnecessary interruptions.