Objective To explore the multiple mediating effects of perceived social support and resilience in the impact mechanism of burnout on depression among clinical nurses, so as to provide references for conducting intervention to improve nurses′ mental health.Methods Based on the Conservation of Resources Theory, a questionnaire survey was conducted among 1,574 clinical nurses by using the Maslach Burnout Inventory-General Survey (MBI-GS), the Multidimensional Scale of Perceived Social Support (MSPSS), the Connor-Davidson Resilience Scale (CD-RISC) and the 9-item Patient Health Questionnaire (PHQ-9) to construct and test a chain mediation model.Results The participants′ total score of MBI-GS, MSPSS, CD-RISC and PHQ-9 was 2.19（1.33,3.02）,71.00（59.00,80.00）, 30.00（22.00,34.00） and 8.00（2.00,10.00）.The score of MBI-GS was negatively correlated with the scores of MSPSS and CD-RISC, and positively correlated with the score of PHQ-9; and the score of MSPSS was negatively correlated with the scores of CD-RISC and PHQ-9 ( all P＜0.05).The total effect of burnout on depression was 0.441, and perceived social support and resilience partially mediated the impact of burnout on depression, making up 11.56% and 14.74% of the mediation effects, respectively, with their combined chain mediation effect accounting for 14.51% of the total effect.Conclusion Negative psychological issues such as burnout and depression are still relatively severe among clinical nurses.Perceived social support and resilience play a chain mediating role between burnout and depression.It is suggested to enhance clinical nurses′ perceived social support and resilience level, so as to mitigate the impact of burnout on depression, finally improve their mental health.