Objective To summarize perioperative nursing experience for patients undergoing Descemet Membrane Endothelial Keratoplasty （DMEK）. Methods Eighteen patients (18 eyes) with endothelial dysfunction underwent DMEK, they were provided sufficient perioperative care and specialized care to improve postoperative complications. Results The surgery for the 18 patients went smoothly, their length of hospitalization was 6-12 (8.17±1.82) days. Fourteen patients experienced ocular hypertension 2 hours after the DMEK, and their intraocular pressure decreased after releasing part anterior chamber air and position care. Five patients (27.8%) suffered from partial detachment of the donor tissue after surgery, and they all completely restored after anterior chamber air reinjection. During 3 months of follow-up, the graft was in place and attached well, without rejection or endothelial dysfunction compensation. At one month after the DMEK, 14 patients had a best-corrected visual acuity of 0.5 or better, and the other 4 patients reached 0.2-0.5. Conclusion It is significant to strengthen perioperative nursing, pay attention to postoperative position control and ocular hypertension management for patients undergoing DMEK, so as to ensure surgery effect.