Objective To explore the application effect of a lactation stage nursing program based on co-parenting concept in breastfeeding for puerperae after cesarean section.Methods A total of 200 pairs of puerperae underwent cesarean section and their spouses were divided into a control group and an experimental group chronologically, with 100 pairs in each group.The control group received routine nursing, while the experimental group received a lactation-stage nursing program based on the co-parenting concept.The lactation process was divided into four stages: a breast differentiation stage, a breast activation stage, a lactation establishment stage, and a lactation maintenance stage, and targeted educational topics and intervention were provided accordingly in each stage.Results Ninety-five pairs and ninety-seven pairs of participants in the control group and experimental group completed the whole study and follow-up. In the experimental group, the score for first breastfeeding and the onset time of maternal lactogenesis were significantly higher and earlier than those in the control group (both P＜0.05).Moreover, neonatal fathers′ level of breastfeeding knowledge, rate of pure breastfeeding at discharge, and the total score as well as each subscale score of the Partner Breast Feeding Influence Scale at six weeks postpartum in the experimental group were significantly higher than those in the control group (all P＜0.05).Conclusion The lactation stage nursing based on co-parenting concept can enhance initial breastfeeding score and neonatal fathers′ level of breastfeeding knowledge, shorten the onset time of maternal lactogenesis, increase pure breastfeeding rates at discharge,and improve puerperae′s perception of spouse′s breastfeeding support, thereby promote breastfeeding.