Objective To investigate the hospice care attitude and hospice care behavior of clinical medical staff, so as to explore the influencing factors of hospice care behavior. Methods A total of 425 clinical staff were investigated with Frommelt Attitude Toward Care of the Dying Scale and Hospice Behavior Measurement Scale.Results The hospice behavior score of clinical staff was (48.00±10.14), which was significantly lower than that of medical staff in hospice institutions (P＜0.05).The hospice attitude score was (103.72±10.70).Hospice attitude was positively correlated with hospice behavior (P＜0.05).The results of stratified regression analysis showed that the level of hospice care behavior was higher in male, married, and specialized hospice care education.The dimension of caring consciousness centered on patients and their families could independently explain 11.6% of the total variation in hospice care behavior.Conclusions Hospice care attitude of clinical staff is generally biased, and hospice care behavior needs to be further improved.Hospice care attitude plays an important role in promoting hospice care behavior.Hospice care behavior of medical staff with different characteristics is different.Hospital managers should carry out professional training to improve the hospice care behavior ability of medical staff.