Objective To deeply understand pediatric nurses′ perception of transition readiness in adolescents with chronic diseases, and to provide reference for transition care practice.Methods Using a descriptive qualitative research method, 13 pediatric nurses were purposively selected and received semi-structured in-depth interviews.Content analysis was employed to analyze and extract themes from the data.Results Four themes were identified:nurses had cognitive deviations in transition readiness, acknowledgement of the importance of transition readiness, barriers to transition readiness (excessive parental disease management, negative emotions of the patient, panic over the dissolution of the attachment relationship, and multiple obstacles to administering transition readiness interventions to the patient), and facilitators of transition readiness (conducting transition preparation training, family and peer support, and providing organizational support).Conclusion Transition readiness is essential for pediatric patients with chronic diseases. However, pediatric nurses have insufficient awareness of transition preparation, and there are numerous barriers to clinical intervention.Managers should strengthen training, establish transition mechanisms, and implement transition preparation intervention, in an effort to help the pediatric patient to achieve a better transition.