Objective To form an expert consensus report on the safe use and maintenance of da Vinci Surgical System (DVSS), and provide practical guidance for standardized use of such instruments. Methods Through literature retrieval and group discussions, the key aspects in operations of DVSS were determined, and the first draft of the expert consensus on the safe use and maintenance of DVSS was formulated.Then an expert consultation form was designed, and used in 2 rounds of Delphi expert consultation.By combining objective evidence and expert opinions, the first draft was revised and refined. Results Expert consensus on the safe use and maintenance of DVSS proposes implementation of standardized surgical nursing from three aspects:terminology, installation and use,and maintenance of the DVSS. Conclusion This expert consensus can provide clinical practice guidance for nurses participating in robot-assisted surgeries, which is conducive to ensuring the safety of patients undergoing surgery and improving the quality of specialized nursing in the operating room.