Objective To explore the effect of evidence-based video-assisted distraction during anesthesia induction on cooperation level of preschool children. Methods We systematically retrieved publications on video-assisted distraction during anesthesia induction and appraised the quality of the publications. Then we assessed the quality of extracted evidence with considerations of clinical scenarios and professional judgment, before we decided to put video-assisted distraction into anesthesia induction practice. Afterwards we selected 82 pre-school children who were to receive an elective surgery and randomized the children into 2 groups. The control group (n=40) received routine preoperative care, while the intervention group (n=42) was additionally subjected to an evidence-based video-assisted distraction program. Children′s preoperative anxiety levels and cooperation levels during anesthesia induction were compared between the two groups. Results After intervention, preoperative anxiety scores and cooperation scores during anesthesia induction in the intervention group were significantly lower than those in the control group (P<0.05,P<0.01). Conclusion The evidence-based video-assisted distraction program can improve cooperation level during anesthesia induction in pre-school children，lessen their anxiety levels, and promote the standard and process of quality nursing service in hospital.